1.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 18(3): 291-3, 2004 Sep.
Artigo
em Chinês
| MEDLINE
| ID: mdl-15640872
RESUMO
OBJECTIVE: To develop a method for detection of coxsackie B virus type 1-6 by RT-PCR. METHODS: A pair of primers were designed to amplify all types of coxsackie B virus 1-6 efficiently. The PCR product was hybridized in micro-wells in which 6 type specific oligonucleotide probes had been coated respectively, colorimetric detection was performed to discriminate the types of coxsackie B virus. RESULTS: This method was shown to be concordant with the IgM ELISA, 71.7% of anti-coxsackie B positive cases could be detected by RT-PCR. CONCLUSION: The RT-PCR method can type coxsackie B virus efficiently and provides a tool for clinical diagnosis and epidemiological investigation.